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1. Use of biology in industrial process and for improving quality of life is called:
A. Biotechnology
B. Eugenics
C. Microbiology
D. Genetic engineering
2. Who discovered recombinant DNA (rDNA ) technology?
A. James D. Watson
B. Walter Sutton and Avery
C. Stanley Cohen and Herbert Boyer
D. Har Gobind Khorana
3. Restriction endonuclease
A. Cuts the DNA molecule at specific sites
B. Synthesizes DNA
C. Restricts the synthesis of DNA inside the nucleus
D. Cuts the DNA molecule randomly
4. Plasmid is a:
A. Extra chromosomal DNA in bacterial cell
B. Fungus
C. Plastid
D. Part of plasma membrane
5. One of the key factors, which makes plasmid the vector in genetic engineering is that
A. It is resistant to antibiotics.
B. It is resistant to restriction enzymes.
C. It has the ability to carry a foreign gene.
D. It has the ability to cause infection in the host.
6. For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of :
A. Silver or Platinum
B. Gold or Tungsten
C. Silicon or Platinum
D. Platinum or Zinc
7. Two microbes found to be very useful in genetic engineering are
A. Escherichia coli and Agrobacterium tumefaciens
B. Vibrio cholerae and a tailed bacteriophage
C. Diplococcus sp. and Pseudomonas sp.
D. Crown gall bacterium and Caenorhabditis elegans
8. Gel electrophoresis is used for
A. Construction of recombinant DNA by joining with cloning vectors
B. Isolation of DNA molecules
C. Separation of DNA fragments according to their size
D. Cutting of DNA into fragments
9. Which one of the following techniques made it possible to genetically engineer living organisms?
A. X-ray diffraction
B. recombinant DNA technique
C. heavier isotope labelling
D. hybridization
10. Which one of the following is a case of wrong matching?
A. Vector DNA - Site for t-RNA synthesis
B. Somatic hybridisation - Fusion of two diverse cells
C. Callus - Unorganised mass of cell produced in tissue culture
D. Micropropagation - In vitro production of plants in large numbers
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